Purification and Characterization of the Glucose Oxidase from Penicillium notatum
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چکیده
منابع مشابه
Enhanced Production of Glucose Oxidase Using Penicillium notatum and Rice Polish
Glucose oxidase (GOD) is an important enzyme that finds a wide range of applications in food and pharmaceutical industry. In this investigation the feasibility of using rice polish as a substrate for the production of GOD by Penicillium notatum in submerged fermentation (SmF) has been evaluated. The intention was to enhance total GOD activity by the selection of economical substrate, microorgan...
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This paper is the second of a series dealing with the analysis of penicillin by means of a chromatogram using solvent-phosphate buffer partition on a silica base. The first paper (Levi, 1948) described the development of this type of chromatogram which is based on that ofMartin & Synge (1941), and outlined the principles of its application to the study of penicillin. The work described was carr...
متن کاملMutarotase from Penicillium notatum. I. Purification, assay, and general properties of the enzyme.
In a study of the mechanism of action of notatin, the flavin adenine dinucleotide-dependent glucose aerodehydrogenase of molds, Bentley and Neuberger (2) in 1949 obtained evidence suggesting that the enzyme preparation also catalyzed the mutarotation of glucose. Keilin and Hartree (3, 4) demonstrated that some notatin samples contained a second enzyme, named mutarotase, which was the most activ...
متن کاملIsolation, purification, and characterization of the PR oxidase from penicillium roqueforti
The PR oxidase, an extracellular enzyme, involved in the conversion of PR toxin into PR acid, was purified from the culture broth of Penicillium roqueforti ATCC 48936. The enzyme has a pI of 4.5 and a molecular mass of approximately 88 kDa, and it is a monomer. The optimum pH for this enzyme is ca. 4.0, and the optimum temperature is 50 degreesC.
متن کاملPurification of phospholipase B from Penicillium notatum by hydrophobic chromatography on palmitoyl cellulose.
Phospholipase B (lysolecithin acyl-hydrolase, EC 3.1.1.5) from the mycelia of Penicillium notatum (Institute for Fermentation, Osaka, Japan; No.4640) was adsorbed from a crude solution to palmitoyl cellulose. Adsorption was efficient at pH 4 at low ionic strength (10 mM buffer), and at pH 4-9 at higher ionic strength (1-2M NaCl in 10 mM buffer). The adsorbed enzyme was eluted from the cellulose...
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ژورنال
عنوان ژورنال: International Journal of Pharmaceutical Quality Assurance
سال: 2018
ISSN: 0975-9506
DOI: 10.25258/ijpqa.v9i01.11360